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Título: | Overexpression of miR-17-5p may negatively impact p300/CBP factor associated inflammation in a hypercholesterolemic advanced prostate cancer model |
Autor(es): | Pimenta, Ruan Camargo, Juliana A. Gonçalves, Guilherme L. Ghazarian, Vitória Candido, Patrícia Guimarães, Vanessa R. Romão, Poliana Chiovatto, Caroline Silva, Karina Serafim da Santos, Gabriel A. dos Silva, Iran A. Nahas, William C. Leite, Kátia R. Pessoa, Ana Flávia Marçal Viana, Nayara I. Reis, Sabrina T. |
Palavras-chave: | Neoplasias da próstata MicroRNAs Inflamação Interleucina-6 Potenciais evocados P300 |
Data do documento: | 2023 |
Editor: | Springer |
Citação: | Pimenta, Ruan, et al. “Overexpression of miR-17-5p may negatively impact P300/CBP factor-associated inflammation in a hypercholesterolemic advanced prostate cancer model”. Molecular Biology Reports, vol. 50, no 9, setembro de 2023, p. 7333–45. |
Resumo: | Background Previously, we demonstrated that cholesterol triggers the increase in p300/CBP-associated factor (PCAF), targeted by miR-17-5p. The p300, IL-6, PCAF, and miR-17-5p genes have important and contradictory roles in inflamma tion and prostate cancer (PCa). This study aimed to demonstrate the potential anti-inflammatory effect of miR-17-5 in an advanced PCa model with diet-induced hypercholesterolemia. Methods and results In vitro, using the PC-3 cell line, we show that induction of miR-17-5p reduces p300 and PCAF expres sion, increases apoptosis, and decreases cell migration. Furthermore, we demonstrate that supplementing this same cell with cholesterol (2 µg/mL) triggers increased p300, IL-6, and PCAF. In vivo, after establishing the hypercholesterolemic (HCOL) model, xenografts were treated with miR-17-5p. Increased expression of this miR after intratumoral injections attenuated tumor growth in the control and HCOL animals and reduced cell proliferation. Conclusion Our results demonstrate that inducing miR-17-5p expression suppresses tumor growth and inflammatory media tor expression. Further studies should be conducted to fully explore the role of miR-17-5p and the involvement of inflamma tory mediators p300, PCAF, and IL-6. |
URI: | http://repo.saocamilo-sp.br:8080/jspui/handle/123456789/1938 |
ISSN: | 1573-4978 |
Aparece nas coleções: | Artigos de Periódicos |
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